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Introducing the RNAscope™ HiPlex V2 assay for single cell and spatial transcriptomic profiling of complex tissues using FFPE and frozen samples

July 27, 2021 @ 10am PST| 1pm EST | 7pm CEST

This webinar will demonstrate detection and characterization of tumor infiltrating immune cells using the new HiPlex V2 assay thereby advancing our understanding of the dynamic crosstalk within the complex and heterogeneous tumor microenvironment (TME).

The RNAscope™ in situ hybridization (ISH) technology provides a powerful method to detect gene expression with spatial and morphological context. The proprietary “double Z” probe design in combination with the advanced signal amplification enables highly specific and sensitive detection of the target RNA with each dot representing a single RNA transcript. The new HiPlex V2 assay expands the capabilities of the existing HiPlex assay and can now be used for simultaneous detection of 12 targets in FFPE tissues in addition to fixed frozen and fresh frozen tissues. 

Learning Objectives:
  • Learn about the expanded capabilities and applications of the HiPlex V2 assay.
  • Understand how the assay enables characterization of immune cell types and activation states.
  • Study complex spatial relationships among different cell types within the TME.


SPEAKER

Anushka

Anushka Dikshit, Ph.D.
Applications Scientist, Genomics
Advanced Cell Diagnostics, a Bio-Techne brand


Purvi Shah

Purvi Shah
Sr. Product Manager, Genomics
Advanced Cell Diagnostics, a Bio-Techne Brand

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