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ACD | University of Wisconsin - Using RNAscope™ Technology for Spatial Resolution

March 10, 2021 @ 10am PST | 1pm EST | 7pm CEST

Incorporating RNAscope to spatially resolve gene expression and validate high throughput data

The RNAscope™ in situ hybridization (ISH) technology provides a powerful method to detect gene expression within the spatial and morphological tissue context. The proprietary “double Z” probe design in combination with the advanced signal amplification enables highly specific and sensitive detection of the target RNA with each dot visualizing a single RNA transcript. This robust signal-to-noise technology allows for the detection of gene transcripts at the single molecule level with single-cell resolution and can further expand our understanding of gene expression in cell lines and tissues samples.

Key Learning Objectives:

  • Validate and visualize target gene expression with spatial context
  • Identify and characterize novel cell populations in complex tissues
  • Visualize RNA and protein targets simultaneously to identify cell-type specific gene expression


SPEAKER

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Anushka Dikshit, Ph.D.
Applications Scientist
Advanced Cell Diagnostics, a Bio-Techne brand

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